Pbad202/d-topo cloning kit
SpletTOPO cloning. Topoisomerase-based cloning (TOPO cloning) is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement for DNA ligases. Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3'-end of the PCR products. This ... SpletCharacterisation of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth. Christopher R Beckwith1, Marcus J Edwards1, Matthew Lawes1, Liang Shi2, Julea N Butt1, David J Richardson1, Thomas A Clarke1*. 1Centre for Molecular and Structural Biochemistry, School of Biological Sciences and …
Pbad202/d-topo cloning kit
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SpletThe pBAD Directional TOPO® Expression Kits offer efficient cloning of blunt-ended PCR products generated with proof-reading DNA polymerases. A choice of ampicillin … Splet17. jul. 2012 · Presentation Transcript. TOPO CLONING By IdiatuBalogun. INTRODUCTION • The structure of DNA is a double-stranded helix, where in the four bases, adenine, thymine, guanine, and cytosine are paired and stored in the centre of this helix. While this structure provides a stable means of storing the genetic code, Watson and Crick noted that the ...
SpletVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
SpletpENTR/D-TOPO 克隆试剂盒利用高效的 5 分钟克隆策略（TOPO 克隆）将平末端 PCR 产物定向克隆成为进入 Gateway 系统或 MultiSite Gateway 系统的载体。平末端 PCR 产物以高 … http://www.veterinaryworld.org/Vol.14/June-2024/35.pdf
Splet三、PCR定向（PCR-Directional）TOPO克隆. 定向TOPO克隆使得克隆PCR产物和其它的DNA分子更加快速和有效。进行5分钟的简单连接，产生>90%的重组子。您不仅会比用连接酶介导的方法更快的获得克隆，而且可以节省因第一次无结果而重复试验所额外浪费的时间 …
http://huayueyang.com.cn/product/277322598 iceage earth tourSpletThe AraC gene product encoded on the pBAD202/D-TOPO ® vector positively regulates this promoter. pBAD202/ D-TOPO ® Vector . pBAD202/D-TOPO ® is designed to facilitate rapid, directional TOPO ® Cloning of blunt-end PCR products for regulated expression in E. coli. Features of the vector include: araBAD promoter (P. BAD) for tight, dose ... ice age every 10000 yearsSpletThe AraC gene product encoded on the pBAD202/D-TOPO vector positively regulates this promoter. pBAD202/D-TOPO载体特征. pBAD202/D-TOPO is designed to facilitate rapid, directional TOPO Cloning of blunt-end PCR products for regulated expression in E. coli. Features of the vector include: araBAD promoter (PBAD) for tight, dose-dependent ... money market codeSpletThe pENTRD-TOPO Cloning Kits utilize a highly efficient, 5-minute cloning strategy (TOPO Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the … iceage empty bottleSpletpENTR™⁄D-TOPO™ Cloning Kit contains pENTR⁄D-TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot™ TOP10 Chemically E. coli, … ice age ellie and baby peachesSpletHere we report the isolation, cloning, and characterization of a novel human Nedd8-specific protease called DEN1. Human DEN1 is encoded by AAH31411.1, a previously … money market companiesSpletis required for cloning into the pBAD202/D TOPO® vector (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. The polymerase chain reaction (PCR) mixture for both the full-length and the 35 kDa fragment . VP2. genes was composed of 20 µL of 5× Phusion HF Buffer, 2 µL of 10 mM dNTPs, 1 µL of each forward and reverse ... ice age filmaffinity